
Skulltulas appear in the Ghost Ship dungeon. However, this idea would later be used for Skulltulas appearing in The Legend of Zelda: Skyward Sword.

Interestingly, in an early demo at the Electronic Entertainment Expo, Skulltulas were shown to be able to tie Link up in a web and attack, but this was cut from the final product. Once Link learns the Back Slice, it can be used to great effect against Skulltulas. Once stunned, Skulltulas can be defeated with the Ending Blow. However, they can easily be dispatched by attacking their heads, either with stabs of a sword or with basic, non-jump wolf attacks. Also, their exoskeletons offer much more protection than before. They attack by crawling towards Link and using a biting attack. Skulltulas have a different attack pattern in Twilight Princess. “It's protected by a hard shell, but its stomach is soft! ” In both games, Skulltulas are able to perform a spin attack when Link approaches them. However, the first regular enemy encountered in the game is a Skulltula, which appears inside the Bombers' Hideout. They are not as frequently encountered as in Ocarina of Time, and invisible ones are encountered only in a single room.

In Majora's Mask, Skulltulas' front sides are invulnerable to Hookshot strikes however, they can still be dispatched with the Hero's Bow. A number of Skulltulas are invisible and can be seen only by using the Lens of Truth.

In Ocarina of Time, the backsides of Skulltulas are vulnerable to sword strikes they can also be defeated if Link hits one with his Hookshot or by shooting it using an arrow with his Fairy Bow.
